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Development of a steroidogenic factor 1/Cre transgenic mouse line
Author(s) -
Bingham Nathan C.,
VermaKurvari Sunita,
Parada Luis F.,
Parker Keith L.
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20231
Subject(s) - transgene , biology , steroidogenic factor 1 , gene knockin , somatic cell , transgenesis , microbiology and biotechnology , gene , cre recombinase , genetically modified mouse , bacterial artificial chromosome , genetics , transcription factor , embryogenesis , nuclear receptor , genome , reproductive biology
The Cre‐loxP strategy provides an approach to disrupt genes in specific tissues and/or cell types, circumventing lethality associated with global knockouts or secondary effects due to gene inactivation at other sites. A critical component is the development of transgenes that target Cre expression to specific cell types. Here, we describe the use of bacterial artificial chromosome (BAC) transgenesis to target Cre expression to tissues that express steroidogenic factor 1 (SF‐1, officially designated Nr5a1). Consistent with the SF‐1 expression pattern, the SF‐1 BAC directed Cre expression to the somatic cells of the gonads, the adrenal cortex, the anterior pituitary, the spleen, and the ventromedial hypothalamic nucleus. This transgene provides a powerful tool to inactivate genes of interest in these tissues. genesis 44:419–424, 2006. Published 2006 Wiley‐Liss, Inc.