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Generation of mice harboring a Sox6 conditional null allele
Author(s) -
Dumitriu Bogdan,
Dy Peter,
Smits Patrick,
Lefebvre Véronique
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20210
Subject(s) - testis determining factor , null allele , biology , allele , conditional gene knockout , genetics
Sox6 belongs to the family of Sry‐related HMG box transcription factors, which determine cell fate and differentiation in various lineages. Sox6 is expressed in several tissues, including cartilage, testis, neuronal, and erythropoietic tissues. Mice lacking Sox6 have revealed critical roles for Sox6 in several of these tissues, but their multiple defects and early lethality has limited studies in specific cell types and in postnatal mice. We show here that we have generated mice harboring a Sox6 conditional null allele ( Sox6 fl+ ) by flanking the second coding exon with loxP sites. This allele encodes wildtype Sox6 protein, is expressed normally, and is efficiently converted into a null allele ( Sox6 fl− ) by Cre‐mediated recombination in somatic and germ cells. Sox6 fl+/fl+ mice are indistinguishable from wildtype mice, and Sox6 fl−/fl− mice from Sox6 −/− mice. These Sox6 conditional null mice will thus be valuable for further uncovering the roles of Sox6 in various processes in vivo. genesis 44:219–224, 2006. Published 2006 Wiley‐Liss, Inc.