Premium
Misexpression screen for genes altering the olfactory map in Drosophila
Author(s) -
Zhang Dongsheng,
Zhou Weiguang,
Yin Chong,
Chen Weitao,
Ozawa Rie,
Ang LayHong,
Anandan Lavanya,
Aigaki Toshiro,
Hing Huey
Publication year - 2006
Publication title -
genesis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.093
H-Index - 110
eISSN - 1526-968X
pISSN - 1526-954X
DOI - 10.1002/dvg.20202
Subject(s) - biology , gene , microbiology and biotechnology , olfactory system , genetic screen , neuroscience , genetics , phenotype
Despite the identification of a number of guidance molecules, a comprehensive picture has yet to emerge to explain the precise anatomy of the olfactory map. From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes. These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling. We analyzed Akap200 , which encodes a Protein Kinase A‐binding protein. Overexpression of Akap200 resulted in fusion of the glomeruli, while its loss resulted in misshapen and ectopic glomeruli. The requirement of Akap200 validates our screen as an effective approach for recovering genes controlling glomerular map patterning. Our finding of diverse classes of genes reveals the complexity of the mechanisms that underlie olfactory map development. genesis 44:189–201, 2006. Published 2006 Wiley‐Liss, Inc.