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Isl‐1 positive pharyngeal mesenchyme subpopulation and its role in the separation and remodeling of the aortic sac in embryonic mouse heart
Author(s) -
Wang JingJing,
Liu HuiXia,
Song Li,
Li HaiRong,
Yang YanPing,
Zhang Tao,
Jing Ya
Publication year - 2019
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.68
Subject(s) - mesenchyme , anatomy , biology , aorta , medicine , embryo , microbiology and biotechnology
Background Second heart field cells and neural crest cells have been reported to participate in the morphogenesis of the pharyngeal arch arteries (PAAs); however, how the PAAs grow out and are separated from the aortic sac into left and right sections is unknown. Results An Isl‐1 positive pharyngeal mesenchyme protrusion in the aortic sac ventrally extends and fuses with the aortic sac wall to form a midsagittal septum that divides the aortic sac. The aortic sac division separates the left and right PAAs to form independent arteries. The midsagittal septum dividing the aortic sac has a different expression pattern from the aortic‐pulmonary (AP) septum in which Isl‐1 positive cells are absent. At 11 days post‐conception (dpc) in a mouse embryo, the Isl‐1 positive mesenchyme protrusion appears as a heart‐shaped structure, in which subpopulations with Isl‐1+ Tbx3+ and Isl‐1+ Nkx2.5+ cells are included. Conclusions The aortic sac is a dynamic structure that is continuously divided during the migration from the pharyngeal mesenchyme to the pericardial cavity. The separation of the aortic sac is not complete until the AP septum divides the aortic sac into the ascending aorta and pulmonary trunk. Moreover, the midsagittal septum and the AP septum are distinct structures.