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Single‐cell analysis of nonhuman primate preimplantation development in comparison to humans and mice
Author(s) -
Hu Youjin,
Huang Kevin,
Zeng Qiao,
Feng Yun,
Ke Qiong,
An Qin,
Qin LianJu,
Cui YuGui,
Guo Ying,
Zhao Dicheng,
Peng Yu,
Tian Di,
Xia Kun,
Chen Yong,
Ni Bin,
Wang Jinmei,
Zhu Xianmin,
Wei Lai,
Liu Yizhi,
Xiang Peng,
Liu JiaYin,
Xue Zhigang,
Fan Guoping
Publication year - 2021
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.295
Subject(s) - biology , gene knockdown , transcriptome , gene , genetics , embryo , zebrafish , wnt signaling pathway , maternal to zygotic transition , morpholino , gene expression profiling , gene regulatory network , embryogenesis , gene expression , zygote
Background Genetic programs underlying preimplantation development and early lineage segregation are highly conserved across mammals. It has been suggested that nonhuman primates would be better model organisms for human embryogenesis, but a limited number of studies have investigated the monkey preimplantation development. In this study, we collect single cells from cynomolgus monkey preimplantation embryos for transcriptome profiling and compare with single‐cell RNA‐seq data derived from human and mouse embryos. Results By weighted gene‐coexpression network analysis, we found that cynomolgus gene networks have greater conservation with human embryos including a greater number of conserved hub genes than that of mouse embryos. Consistently, we found that early ICM/TE lineage‐segregating genes in monkeys exhibit greater similarity with human when compared to mouse, so are the genes in signaling pathways such as LRP1 and TCF7 involving in WNT pathway. Last, we tested the role of one conserved pre‐EGA hub gene, SIN3A, using a morpholino knockdown of maternal RNA transcripts in monkey embryos followed by single‐cell RNA‐seq. We found that SIN3A knockdown disrupts the gene‐silencing program during the embryonic genome activation transition and results in developmental delay of cynomolgus embryos. Conclusion Taken together, our study provided new insight into evolutionarily conserved and divergent transcriptome dynamics during mammalian preimplantation development.