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The Drosophila CG1674 gene encodes a synaptopodin 2‐like related protein that localizes to the Z‐disc and is required for normal flight muscle development and function
Author(s) -
Czajkowski Emily R.,
Cisneros Marilyn,
Garcia Bianca S.,
Shen Jim,
Cripps Richard M.
Publication year - 2021
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.250
Subject(s) - gene knockdown , myofibril , biology , microbiology and biotechnology , cytoplasm , actin , desmin , actinin , gene , anatomy , genetics , biochemistry , cytoskeleton , cell , vimentin , immunohistochemistry , immunology
Background To identify novel myofibrillar components of the Drosophila flight muscles, we carried out a proteomic analysis of chemically demembranated flight muscle myofibrils, and characterized the knockdown phenotype of a novel gene identified in the screen, CG1674 . Results The CG1674 protein has some similarity to vertebrate synaptopodin 2‐like, and when expressed as a FLAG‐tagged fusion protein, it was localized during development to the Z‐disc and cytoplasm. Knockdown of CG1674 expression affected the function of multiple muscle types, and defective flight in adults was accompanied by large actin‐rich structures in the flight muscles that resembled overgrown Z‐discs. Localization of CG1674 to the Z‐disc depended predominantly upon presence of the Z‐disc component alpha‐actinin, but also depended upon other Z‐disc components, including Mask, Zasp52, and Sals. We also observed re‐localization of FLAG‐CG1674 to the nucleus in Alpha‐actinin and sals knockdown animals. Conclusions These studies identify and characterize a previously unreported myofibrillar component of Drosophila muscle that is necessary for proper myofibril assembly during development.