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Embryogenesis and early skeletogenesis in the antarctic bullhead notothen, Notothenia coriiceps
Author(s) -
Postlethwait John H.,
Yan Yilin,
Desvignes Thomas,
Allard Corey,
Titus Tom,
Le François Nathalie R.,
Detrich H. William
Publication year - 2016
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.24437
Subject(s) - biology , epiboly , hatching , embryogenesis , spawn (biology) , yolk sac , gastrulation , zebrafish , human fertilization , ecology , embryo , zoology , fishery , anatomy , genetics , gene
Background: Environmental temperature influences rates of embryonic development, but a detailed staging series for vertebrate embryos developing in the subzero cold of Antarctic waters is not yet available from fertilization to hatching. Given projected warming of the Southern Ocean, it is imperative to establish a baseline to evaluate potential effects of changing climate on fish developmental dynamics. Results: We studied the Bullhead notothen ( Notothenia coriiceps ), a notothenioid fish inhabiting waters between ‐1.9 and +2 °C. In vitro fertilization produced embryos that progressed through cleavage, epiboly, gastrulation, segmentation, organogenesis, and hatching. We compared morphogenesis spatially and temporally to Zebrafish and medaka. Experimental animals hatched after about 6 months to early larval stages. To help understand skeletogenesis, we analyzed late embryos for expression of sox9 and runx2 , which regulate chondrogenesis, osteogenesis, and eye development. Results revealed that, despite their prolonged developmental time course, N. coriiceps embryos developed similarly to those of other teleosts with large yolk cells. Conclusions: Our studies set the stage for future molecular analyses of development in these extremophile fish. Results provide a foundation for understanding the impact of ocean warming on embryonic development and larval recruitment of notothenioid fish, which are key factors in the marine trophic system. Developmental Dynamics 245:1066–1080, 2016 . © 2016 Wiley Periodicals, Inc.