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Cdk5 regulates developmental remodeling of mushroom body neurons in Drosophila
Author(s) -
SmithTrunova Svetlana,
Prithviraj Ranjini,
Spurrier Joshua,
Kuzina Irina,
Gu Qun,
Giniger Edward
Publication year - 2015
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.24350
Subject(s) - mushroom bodies , biology , drosophila (subgenus) , microbiology and biotechnology , mushroom , cyclin dependent kinase 5 , genetics , drosophila melanogaster , gene , signal transduction , botany , mitogen activated protein kinase kinase , protein kinase c
Background: During metamorphosis, axons and dendrites of the mushroom body (MB) in the Drosophila central brain are remodeled extensively to support the transition from larval to adult behaviors. Results: We show here that the neuronal cyclin‐dependent kinase, Cdk5, regulates the timing and rate of mushroom body remodeling: reduced Cdk5 activity causes a delay in pruning of MB neurites, while hyperactivation accelerates it. We further show that Cdk5 cooperates with the ubiquitin‐proteasome system in this process. Finally, we show that Cdk5 modulates the first overt step in neurite disassembly, dissolution of the neuronal tubulin cytoskeleton, and provide evidence that it also acts at additional steps of MB pruning. Conclusions: These data show that Cdk5 regulates the onset and extent of remodeling of the Drosophila MB. Given the wide phylogenetic conservation of Cdk5, we suggest that it is likely to play a role in developmental remodeling in other systems, as well. Moreover, we speculate that the well‐established role of Cdk5 in neurodegeneration may involve some of the same cellular mechanisms that it uses during developmental remodeling. Developmental Dynamics 244:1550–1563, 2015 . © 2015 Wiley Periodicals, Inc.