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Sebox regulates mesoderm formation in early amphibian embryos
Author(s) -
Chen Geng,
Tan Renbo,
Tao Qinghua
Publication year - 2015
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.24323
Subject(s) - mesoderm , biology , fgf and mesoderm formation , gastrulation , nodal , paraxial mesoderm , microbiology and biotechnology , zebrafish , intermediate mesoderm , notochord , nodal signaling , convergent extension , endoderm , wnt signaling pathway , lateral plate mesoderm , xenopus , embryogenesis , morpholino , embryo , genetics , embryonic stem cell , signal transduction , gene
Background: Mix/Bix genes are important regulators of mesendoderm formation during vertebrate embryogenesis. Sebox , an additional member of this gene family, has been implicated in endoderm formation during early embryogenesis in zebrafish. However, it remains unclear whether Sebox plays a unique role in early Xenopus embryos. Results: In this study, we provide evidence that Sebox is uniquely required for the formation of mesoderm during early Xenopus embryogenesis. Sebox is dynamically expressed in the involuted mesoderm during gastrulation. It is activated by Nodal/Activin signaling and modulated by zygotic Wnt/β‐catenin signaling. Overexpression of Sebox perturbs movements during convergent extension and inhibits the expression of mesodermal, but not endodermal, genes induced by Nodal/Activin signaling. Depletion of Sebox using a specific morpholino increases the expression of noncanonical wnt5a , wnt5b , and wnt11b . Depletion of Sebox also up‐regulates the expression of pcdh8.2 , a paraxial mesoderm‐specific protocadherin, in a Wnt11B‐dependent manner. Sebox morphants display reduced development of the head and notochord. Conclusions: Our findings illustrate that Sebox, a unique member of the Mix/Bix gene family, functions downstream of Nodal/Activin signaling and is required for the proper expression of noncanonical Wnt ligands and the normal development of mesoderm in Xenopus . Developmental Dynamics 244:1415–1426, 2015 . © 2015 Wiley Periodicals, Inc.