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Nuclear phosphatase PPM1G in cellular survival and neural development
Author(s) -
Foster William H.,
Langenbacher Adam,
Gao Chen,
Chen Jaunian,
Wang Yibin
Publication year - 2013
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23990
Subject(s) - biology , zebrafish , microbiology and biotechnology , neural tube , programmed cell death , neurulation , neural development , embryo , embryogenesis , genetics , gene , gastrulation , apoptosis
Background: PPM1G is a nuclear localized serine/threonine phosphatase implicated to be a regulator of chromatin remodeling, mRNA splicing, and DNA damage. However, its in vivo function is unknown. Results: Here we show that ppm1g expression is highly enriched in the central nervous system during mouse and zebrafish development. ppm1g −/− mice were embryonic lethal with incomplete penetrance after E12.5. Rostral defects, including neural tube and craniofacial defects were observed in ppm1g −/− embryos associated with increased cell death in the neural epithelium. In zebrafish, loss of ppm1g also led to neural defects with aberrant neural marker gene expression. Primary fibroblasts from ppm1g −/− embryos failed to grow without immortalization while immortalized ppm1g −/− fibroblasts had increased cell death upon oxidative and genotoxic stress when compared to wild type fibroblasts. Conclusions: Our in vivo and in vitro studies revealed a critical role for PPM1G in normal development and cell survival. Developmental Dynamics 242:1101–1109, 2013 . © 2013 Wiley Periodicals, Inc.