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RNA interference by feeding in vitro–synthesized double‐stranded RNA to planarians: Methodology and dynamics
Author(s) -
Rouhana Labib,
Weiss Jennifer A.,
Forsthoefel David J.,
Lee Hayoung,
King Ryan S.,
Inoue Takeshi,
Shibata Norito,
Agata Kiyokazu,
Newmark Phillip A.
Publication year - 2013
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23982
Subject(s) - planarian , biology , rna , rna interference , flatworm , in vitro , microbiology and biotechnology , rna silencing , messenger rna , regeneration (biology) , zoology , biochemistry , gene
COVER PHOTOGRAPH : Low magnification image of planarian flatworms ( Schmidtea mediterranea ) fed with liver paste containing either red, green, yellow, or blue food dye. Uptake of colored liver paste containing in vitro synthesized double‐stranded RNA is readily visualized in the planarian gut. Ingestion and processing of double‐stranded RNA in the planarian gut leads to systemic RNA‐interference. Image credit: Labib Rouhana and Phillip A. Newmark, Howard Hughes Medical Institute and Department of Cell and Developmental Biology, University of Illinois at Urbana‐Champaign. From RNA Interference by feeding in vitro‐synthesized double‐stranded RNA to planarians, Labib Rouhana, Jennifer A. Weiss, David J. Forsthoefel, Hayoung Lee, Ryan S. King, Takeshi Inoue, Norito Shibata, Kiyokazu Agata, and Phillip A. Newmark, Developmental Dynamics 242:718–730, 2013.