Premium
Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development
Author(s) -
FilipekGórniok Beata,
Holmborn Katarina,
Haitina Tatjana,
Habicher Judith,
Oliveira Marta Bastos,
Hellgren Charlotte,
Eriksson Inger,
Kjellén Lena,
Kreuger Johan,
Ledin Johan
Publication year - 2013
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23981
Subject(s) - glycosyltransferase , zebrafish , biology , chondroitin , chondroitin sulfate , gene , biochemistry , dermatan sulfate , microbiology and biotechnology , glycosaminoglycan
Background: Chondroitin/dermatan sulfate (CS/DS) proteoglycans present in the extracellular matrix have important structural and regulatory functions. Results: Six human genes have previously been shown to catalyze CS/DS polymerization. Here we show that one of these genes, chpf , is represented by two copies in the zebrafish genome, chpfa and chpfb , while the other five human CS/DS glycosyltransferases csgalnact1, csgalnact2, chpf2, chsy1, and chsy3 all have single zebrafish orthologues. The putative zebrafish CS/DS glycosyltransferases are spatially and temporally expressed. Interestingly, overlapping expression of multiple glycosyltransferases coincides with high CS/DS deposition. Finally, whereas the relative levels of the related polysaccharide HS reach steady‐state at around 2 days post fertilization, there is a continued relative increase of the CS amounts per larvae during the first 6 days of development, matching the increased cartilage formation. Conclusions: There are 7 CS/DS glycosyltransferases in zebrafish, which, based on homology, can be divided into the CSGALNACT, CHSY, and CHPF families. The overlap between intense CS/DS production and the expression of multiple CS/DS glycosyltransferases suggests that efficient CS/DS biosynthesis requires a combination of several glycosyltransferases. Developmental Dynamics 242:964–975, 2013 . © 2013 Wiley Periodicals, Inc.