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In vitro oocyte culture‐based manipulation of zebrafish maternal genes
Author(s) -
Nair Sreelaja,
Lindeman Robin E.,
Pelegri Francisco
Publication year - 2013
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23930
Subject(s) - biology , zebrafish , cleavage (geology) , embryo , mutant , oocyte , microbiology and biotechnology , gene , in vitro , genetics , anatomy , andrology , paleontology , fracture (geology) , medicine
Images showing wild type cleavage stage zebrafish embryos (left column), cleavage stage embryos carrying the cei mutation (middle), and cie mutant embryos rescued with wild‐type aurB mRNA (right). Green fluorescence visualizes bcatenin, red fluorescence visualizes a‐tubulin. Visualization of both channels is seen in the top row. See Nair et al., Developmental Dynamics 242:44–52 for original figure and additional experimental details.