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Structural disorganization of pronephric glomerulus in Zebrafish mpp5a/nagie oko mutant
Author(s) -
Ichimura Koichiro,
Fukuyo Yayoi,
Nakamura Tomomi,
Powell Rebecca,
Sakai Tatsuo,
Obara Tomoko
Publication year - 2012
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23877
Subject(s) - zebrafish , biology , glomerulus , microbiology and biotechnology , mesangium , slit diaphragm , anatomy , mutant , podocyte , endocrinology , kidney , genetics , glomerulonephritis , gene , proteinuria
Abstract Background: The podocyte slit diaphragm (SD) is an essential component of the selective filtration barrier in the glomerulus. Several structural proteins required for formation and maintenance of SD have been identified; however, molecular mechanisms regulating these proteins are still limited. Results: Here, we demonstrate that MAGUK p55 subfamily member 5a (Mpp5a)/Nagie oko, a component of the Crb multi‐protein complex, was colocalized with an SD‐associated protein ZO‐1 in the zebrafish pronephric glomerulus. To characterize the function of Mpp5a, zebrafish mpp5a m520 mutant embryos, which are known to have defects in cardiac and neuronal morphogenesis, were analyzed. These mutants failed to merge the bilateral glomerular primordia and to form the glomerular capillary and mesangium, but the foot processes and SD showed normal appearance. The structural disorganization in the mpp5a m520 mutant glomerulus was quite similar to that of a cardiac troponin T2a/tnnt2a/silent heart knockdown zebrafish, which exhibited circulatory failure due to lack of heart beating. Conclusions: Mpp5a is not prerequisite to form podocyte slit diaphragm in the pronephric glomerular development in zebrafish. The structural disorganization of the pronephric glomerulus in the mpp5a m520 mutant is likely to result from circulatory failure, rather than the anomaly of Mpp5a protein in the glomerulus. Developmental Dynamics, 2012. © 2012 Wiley Periodicals, Inc.