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Distinct roles of MicroRNAs in epithelium and mesenchyme during tooth development
Author(s) -
Oommen Shelly,
OtsukaTanaka Yoko,
Imam Najam,
Kawasaki Maiko,
Kawasaki Katsushige,
JalaniGhazani Farnoosh,
Anderegg Angela,
Awatramani Rajeshwar,
Hindges Robert,
Sharpe Paul T.,
Ohazama Atsushi
Publication year - 2012
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23828
Subject(s) - biology , mesenchyme , microrna , microbiology and biotechnology , epithelium , anatomy , computational biology , evolutionary biology , genetics , gene
Background: Tooth development is known to be mediated by the cross‐talk between signaling pathways, including Shh, Fgf, Bmp, and Wnt. MicroRNAs (miRNAs) are 19‐ to 25‐nt noncoding small single‐stranded RNAs that negatively regulate gene expression by binding target mRNAs, which is believed to be important for the fine‐tuning signaling pathways in development. To investigate the role of miRNAs in tooth development, we examined mice with either mesenchymal ( Wnt1Cre/Dicer fl/fl ) or epithelial ( ShhCre/Dicer fl/fl ) conditional deletion of Dicer , which is essential for miRNA processing. Results: By using a CD1 genetic background for Wnt1Cre/Dicer fl/fl , we were able to examine tooth development, because the mutants retained mandible and maxilla primordia. Wnt1Cre/Dicer fl/fl mice showed an arrest or absence of teeth development, which varied in frequency between incisors and molars. Extra incisor tooth formation was found in ShhCre/Dicer fl/fl mice, whereas molars showed no significant anomalies. Microarray and in situ hybridization analysis identified several miRNAs that showed differential expression between incisors and molars. Conclusion: In tooth development, miRNAs thus play different roles in epithelium and mesenchyme, and in incisors and molars. Developmental Dynamics 241:1465–1472, 2012. © 2012 Wiley Periodicals, Inc.