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Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum
Author(s) -
Nagy Nandor,
Burns Alan J.,
Goldstein Allan M.
Publication year - 2012
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23801
Subject(s) - neural crest , biology , neural tube , neural fold , chimera (genetics) , embryo , anatomy , enteric nervous system , microbiology and biotechnology , crest , green fluorescent protein , neural plate , sox10 , neuroscience , genetics , gene , physics , quantum mechanics
A chick–chick intraspecies chimera was created by removing the neural tube adjacent to somites 2–6 from a normal chick embryo at E1.5 and replacing it with equivalent tissue from an age‐matched chick‐GFP transgenic embryo. At E10, the colorectum was removed, sectioned, and stained with HNK‐1 antibody (red) to detect neural crest‐derived cells, and with DAPI (blue) to label nuclei. Vagal neural crest‐derived cells are HNK‐1+/GFP+, while sacral neural crest derived‐cells, which comprise the nerve of Remak, are HNK‐1+/GFP−. From Nagy et al., Developmental Dynamics 241:842–851, 2012. © 2012 Wiley Periodicals, Inc.