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Peri‐implantation lethality in mice lacking the PGC‐1‐related coactivator protein
Author(s) -
He Xin,
Sun Chen,
Wang Feng,
Shan Aijing,
Guo Ting,
Gu Weiqiong,
Cui Bin,
Ning Guang
Publication year - 2012
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23769
Subject(s) - biology , lethality , coactivator , microbiology and biotechnology , andrology , peri , genetics , medicine , gene , transcription factor
Background: Members of the PPARγ coactivator‐1 (PGC‐1) family are central transcriptional coactivators that regulate cell metabolic processes ranging from mitochondrial biogenesis to oxidative respiration. PGC‐1‐related coactivator (PPRC1 or PRC), initially identified as a member of the PGC‐1 family, is believed to regulate mitochondria biogenesis, respiration pathways, and cell proliferation. However, its physiological role is not clearly understood. Here, we investigate the biological functions of PPRC1 in vivo using PPRC1 deficient mice generated by gene targeting. Results: Homozygous deficient PPRC1 mice failed to form egg cylinders and died after implantation but before embryonic day 6.5, whereas mice heterozygous for PPRC1 were viable, fertile and indistinguishable from their wild‐type littermates. Furthermore, PPRC1 mRNA was expressed at the embryonic stage before implantation and was rapidly up‐regulated during the first day of embryoid body formation. The PPRC1 mRNA was then subsequently down‐regulated, although its precise function at this stage of development was unclear. Conclusions: This is the first study to suggest a nonredundant role for PPRC1 in mouse early embryonic development. Developmental Dynamics 241:975–983, 2012. © 2012 Wiley Periodicals, Inc.