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Astacin‐like metallo‐endopeptidase is dynamically expressed in embryonic stem cells and embryonic epithelium during morphogenesis
Author(s) -
Acloque Hervé,
Lavial Fabrice,
Pain Bertrand
Publication year - 2012
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.23737
Subject(s) - biology , ectoderm , mesonephros , microbiology and biotechnology , epiblast , embryonic stem cell , embryogenesis , neural fold , neural tube , organogenesis , morphogenesis , gastrulation , mesoderm , stem cell , endoderm , neural crest , embryo , anatomy , neural plate , genetics , gene
Background: Astacin‐like metallo‐proteases are zinc endopeptidases conserved among vertebrates and invertebrates. First described as hatching gland enzymes, many members of the family possess other functions during embryonic development. In the chick, however, functions of Astacin‐like proteins remain elusive. Results: We report here that Astacin‐like ( ASTL ) is strongly expressed in mouse and chicken embryonic stem (ES) cells and exhibits a very dynamic expression pattern during embryogenesis and organogenesis, mostly in remodeled epithelia. Consistent with its expression in ES cells, chick ASTL is detected in vivo in the pluripotent cells of the epiblast and then disappears from the newly induced neural plate. ASTL expression remains at the junction of non‐neural and neural ectoderm, just before neural tube closure. At later stages, chick ASTL is detected in the ventral epidermis before ventral closure, in the intermediate mesoderm, in the gonads and in the forming nephric duct and tubules of the mesonephros and metanephros. Conclusions: ASTL is dynamically expressed in the embryonic epithelium and in embryonic stem cells, suggesting an important function for the control of epithelial cell behavior during early development. Developmental Dynamics 241:574–582, 2012. © 2012 Wiley Periodicals, Inc.