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Cloning and expression of a retinoic acid receptor β2 subtype from the adult newt: Evidence for an early role in tail and caudal spinal cord regeneration
Author(s) -
Carter Christopher,
Clark Alysen,
Spencer Gaynor,
Carlone Robert
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22769
Subject(s) - biology , retinoic acid , spinal cord , regeneration (biology) , retinoic acid receptor , central nervous system , receptor , microbiology and biotechnology , retinoid , ependymal cell , anatomy , in situ hybridization , medicine , endocrinology , neuroscience , messenger rna , genetics , cell culture , gene
Retinoic acid receptor beta 2 (RARβ2) has been proposed as an important receptor mediating retinoid‐induced axonal growth and regeneration in developing mammalian spinal cord and brain. In urodele amphibians, organisms capable of extensive central nervous system (CNS) regeneration as adults, this receptor had not been isolated, nor had its function been characterized. We have cloned a full‐length RARβ2 cDNA from adult newt CNS. This receptor, Nv RARβ2, is expressed in various adult organs capable of regeneration, including the spinal cord. Interestingly, both the Nv RARβ2 mRNA and protein are up‐regulated during the first 2 weeks after amputation of the tail, primarily in the ependymoglial and meningeal tissues near the rostral cut surface of the cord. Treatment with LE135, a RARβ‐selective antagonist, caused a significant inhibition of ependymal outgrowth and a decrease in tail regenerate length. These data support an early role for this receptor in caudal spinal cord and tail regeneration in this amphibian. Developmental Dynamics 240:2613–2625, 2011. © 2011 Wiley Periodicals, Inc.