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Live imaging of endogenous periodic tryptophan protein 2 gene homologue during zebrafish development
Author(s) -
Jayasena Chathurani S.,
Trinh Le A.,
Bronner Marianne
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22744
Subject(s) - biology , zebrafish , ribosome biogenesis , nucleolus , microbiology and biotechnology , endoderm , protein biosynthesis , ribosome , gene , genetics , rna , cytoplasm , cellular differentiation
Yeast Periodic tryptophan protein 2 gene (Pwp2) is involved in ribosome biogenesis and has been implicated in regulation of the cell cycle in yeast. Here, we report a zebrafish protein‐trap line that produces fluorescently tagged Periodic tryptophan protein 2 gene homologue (Pwp2h) protein, which can be dynamically tracked in living fish at subcellular resolution. We identified both full‐length zebrafish Pwp2h and a short variant. The expression results show that Pwp2h is present in numerous sites in the early developing embryo, but later is restricted to highly proliferative regions, including the forebrain ventricular zone and endoderm‐derived organs in the early larval stage. At the subcellular level, Pwp2h protein appears to be localized to the region of the nucleolus consistent with its presumed function in ribosomal RNA synthesis. This Pwp2h protein trap line offers a powerful tool to study the link between ribosome biogenesis and cell cycle progression during vertebrate development. Developmental Dynamics 240:2578–2583, 2011. © 2011 Wiley Periodicals, Inc.