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Hematopoietic prostaglandin D synthase (H‐Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein
Author(s) -
Moniot Brigitte,
Farhat Andalib,
Aritake Kosuke,
Declosmenil Faustine,
Nef Serge,
Eguchi Naomi,
Urade Yoshihiro,
Poulat Francis,
BoizetBonhoure Brigitte
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22726
Subject(s) - biology , sox9 , sertoli cell , gonad , chromosomal translocation , microbiology and biotechnology , medicine , endocrinology , gene expression , spermatogenesis , gene , genetics
Abstract In mammals, the Prostaglandin D 2 (PGD 2 ) signaling pathway is involved in male gonadal development, regulating Sox9 gene expression and SOX9 protein subcellular localization through lipocalin prostaglandin D synthase (L‐Pgds) activity. Nevertheless, because L‐Pgds is downstream of Sox9 , its expression cannot explain the initial nuclear translocation of the SOX9 protein. Here, we show that another source of PGD 2 , hematopoietic‐Pgds (H‐Pgds) enzyme is expressed in somatic and germ cells of the embryonic gonad of both sexes, as early as embryonic day (E) 10.5, before the onset of L‐Pgds expression. Inhibition of H‐Pgds activity by the specific HQL‐79 inhibitor leads to impaired nuclear translocation of SOX9 protein in E11.5 Sertoli cells. Furthermore, analysis of H‐Pgds −/− male embryonic gonads confirms abnormal subcellular localization of SOX9 protein at the E11.5 early stage of mouse testicular differentiation suggesting a role for H‐Pgds‐produced PGD 2 in the initial nuclear translocation of SOX9. Developmental Dynamics 240:2335–2343, 2011. © 2011 Wiley‐Liss, Inc.