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Alkali‐like myosin light chain‐1 ( myl1 ) is an early marker for differentiating fast muscle cells in zebrafish
Author(s) -
Burguière A. C.,
Nord H.,
von Hofsten J.
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22677
Subject(s) - myosin , biology , zebrafish , myogenesis , myosin light chain kinase , immunoglobulin light chain , microbiology and biotechnology , gene , myofibril , myocyte , troponin , genetics , biochemistry , antibody , psychology , psychiatry , myocardial infarction
During myogenesis, muscle precursors become divided into either fast‐ or slow‐twitch fibres, which in the zebrafish occupy distinct domains in the embryo. Genes encoding sarcomeric proteins specific for fast or slow fibres are frequently used as lineage markers. In an attempt to identify and evaluate early definitive markers for cells in the fast‐twitch pathway, we analysed genes encoding proteins contributing to the fast sarcomeric structures. The previously uncharacterized zebrafish alkali‐like myosin light chain gene ( myl1 ) was found to be expressed exclusively in cells in the fast‐twitch pathway initiated at an early stage of fast fibre differentiation. Myl1 was expressed earlier, and in a more fibre type restricted manner, than any of the previously described and frequently used fast myosin light and heavy chain and troponin muscle markers mylz2, mylz3, tnni2, tnnt3a, fMyHC1.3 . In summary, this study introduces a novel marker for early differentiating fast muscle cells. Developmental Dynamics 240:1856–1863, 2011. © 2011 Wiley‐Liss, Inc.