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Cell adhesion molecule cadherin‐6 function in zebrafish cranial and lateral line ganglia development
Author(s) -
Liu Q.,
Dalman M.R.,
Sarmah S.,
Chen S.,
Chen Y.,
Hurlbut A.K.,
Spencer M.A.,
Pancoe L.,
Marrs J.A.
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22665
Subject(s) - biology , zebrafish , lateral line , ganglion , cadherin , anatomy , morpholino , nervous system , embryogenesis , in situ hybridization , neuroscience , microbiology and biotechnology , embryo , gene expression , gene , genetics , cell
Cadherins regulate the vertebrate nervous system development. We previously showed that cadherin‐6 message ( cdh6 ) was strongly expressed in the majority of the embryonic zebrafish cranial and lateral line ganglia during their development. Here, we present evidence that cdh6 has specific functions during cranial and lateral line ganglia and nerve development. We analyzed the consequences of cdh6 loss‐of‐function on cranial ganglion and nerve differentiation in zebrafish embryos. Embryos injected with zebrafish cdh6 specific antisense morpholino oligonucleotides (MOs, which suppress gene expression during development; cdh6 morphant embryos) displayed a specific phenotype, including (i) altered shape and reduced development of a subset of the cranial and lateral line ganglia (e.g., the statoacoustic ganglion and vagal ganglion) and (ii) cranial nerves were abnormally formed. These data illustrate an important role for cdh6 in the formation of cranial ganglia and their nerves. Developmental Dynamics 240:1716–1726, 2011. © 2011 Wiley‐Liss, Inc.