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Control of pelvic girdle development by genes of the Pbx family and Emx2
Author(s) -
Capellini Terence D.,
Handschuh Karen,
Quintana Laura,
Ferretti Elisabetta,
Di Giacomo Giuseppina,
Fantini Sebastian,
Vaccari Giulia,
Clarke Shoa L.,
Wenger Aaron M.,
Bejerano Gill,
Sharpe James,
Zappavigna Vincenzo,
Selleri Licia
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22617
Subject(s) - biology , mesoderm , genetics , homeobox , emx2 , gene , somite , hox gene , mutant , microbiology and biotechnology , embryonic stem cell , gene expression
Genes expressed in the somatopleuric mesoderm, the embryonic domain giving rise to the vertebrate pelvis, appear important for pelvic girdle formation. Among such genes, Pbx family members and Emx2 were found to genetically interact in hindlimb and pectoral girdle formation. Here, we generated compound mutant embryos carrying combinations of mutated alleles for Pbx1 , Pbx2 , and Pbx3 , as well as Pbx1 and Emx2 , to examine potential genetic interactions during pelvic development. Indeed, Pbx genes share overlapping functions and Pbx1 and Emx2 genetically interact in pelvic formation. We show that, in compound Pbx1;Pbx2 and Pbx1;Emx2 mutants, pelvic mesenchymal condensation is markedly perturbed, indicative of an upstream control by these homeoproteins. We establish that expression of Tbx15 , Prrx1 , and Pax1 , among other genes involved in the specification and development of select pelvic structures, is altered in our compound mutants. Lastly, we identify potential Pbx1‐Emx2–regulated enhancers for Tbx15 , Prrx1 , and Pax1 , using bioinformatics analyses. Developmental Dynamics 240:1173–1189, 2011. © 2011 Wiley‐Liss, Inc.