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Exogenous fibroblast growth factor 8 rescues development of mouse diastemal vestigial tooth ex vivo
Author(s) -
Li Lu,
Yuan Guohua,
Liu Chao,
Zhang Lu,
Zhang Yanding,
Chen YiPing,
Chen Zhi
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22596
Subject(s) - biology , fgf8 , fibroblast growth factor , diastema , dentition , microbiology and biotechnology , anatomy , genetics , paleontology , receptor
Regression of vestigial tooth buds results in the formation of the toothless diastema, a unique feature of the mouse dentition. Revitalization of the diastemal vestigial tooth bud provides an excellent model for studying tooth regeneration and replacement. It has been previously shown that suppression of fibroblast growth factor (FGF) signaling in the diastema results in vestigial tooth bud regression. In this study, we report that application of exogenous FGF8 to the mouse embryonic diastemal region rescues diastemal tooth development. However, this rescue of diastemal tooth development occurs only in an isolated diastemal regions and not in the mandibular quadrant, which includes the incisor and molar germs. FGF8 promotes cell proliferation and inhibits apoptosis in diastemal tooth epithelium, and revitalizes the tooth developmental program, as evidenced by the expression of genes critical for normal tooth development. Our results also support the idea that the adjacent tooth germs contribute to the suppression of diastemal vestigial tooth buds by means of multiple signals. Developmental Dynamics 240:1344–1353, 2011. © 2011 Wiley‐Liss, Inc.