Premium
Hts, the Drosophila homologue of adducin, physically interacts with the transmembrane receptor golden goal to guide photoreceptor axons
Author(s) -
Ohler Stephan,
HakedaSuzuki Satoko,
Suzuki Takashi
Publication year - 2011
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22515
Subject(s) - biology , growth cone , marcks , microbiology and biotechnology , filopodia , axon guidance , mutant , transmembrane domain , transmembrane protein , semaphorin , drosophila melanogaster , axon , receptor , neuroscience , actin , genetics , signal transduction , gene , protein kinase c
Neurons steer their axons towards their proper targets during development. Molecularly, a number of guidance receptors have been identified. The transmembrane protein Golden goal (Gogo) was reported previously to guide photoreceptor (R) axons in the Drosophila visual system. Here, we show that Hts, the Drosophila homologue of Adducin, physically interacts with Gogo's cytoplasmic domain via its head‐neck domain. hts null mutants show similar defects in R axon guidance as do gogo mutants. Rescue experiments suggest that the C‐terminal tail but not the MARCKS homology domain of Hts is required. Overexpression of either gogo or hts causes abnormally thick swellings of R8 axons in the medulla, but if both are co‐overexpressed, R8 axons appear normal and the amount of excessive Hts is reduced. Our results fit with a model where Gogo both positively and negatively regulates Hts that affects the Actin‐Spectrin cytoskeleton in growth cone filopodia, thereby guiding R axons. Developmental Dynamics, 2011. © 2010 Wiley‐Liss, Inc.