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The G‐protein‐coupled receptor, GPR84 , is important for eye development in Xenopus laevis
Author(s) -
Perry Kimberly J.,
Johnson Verity R.,
Malloch Erica L.,
Fukui Lisa,
Wever Jason,
Thomas Alvin G.,
Hamilton Paul W.,
Henry Jonathan J.
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22446
Subject(s) - xenopus , biology , morpholino , retina , microbiology and biotechnology , lens (geology) , gene knockdown , cornea , ectoderm , eye development , receptor , anatomy , embryogenesis , neuroscience , embryo , apoptosis , genetics , transcription factor , paleontology , gene
G‐protein‐coupled receptors (GPCRs) represent diverse, multifamily groups of cell signaling receptors involved in many cellular processes. We identified Xenopus laevis GPR84 as a member of the A18 subfamily of GPCRs. During development, GPR84 is detected in the embryonic lens placode, differentiating lens fiber cells, retina, and cornea. Anti‐sense morpholino oligonucleotide‐mediated knockdown and RNA rescue experiments demonstrate GPR84 ' s importance in lens, cornea, and retinal development. Examination of cell proliferation using an antibody against histone H3 S10P reveals significant increases in the lens and retina following GPR84 knockdown. Additionally, there was also an increase in apoptosis in the retina and lens, as revealed by TUNEL assay. Reciprocal transplantation of the presumptive lens ectoderm between uninjected controls and morpholino‐injected embryos demonstrates that GPR84 is necessary in the retina for proper development of the retina, as well as other eye tissues including the lens and cornea. Developmental Dynamics 239:3024–3037, 2010. © 2010 Wiley‐Liss, Inc.