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Expression of GPR177 (Wntless/Evi/Sprinter), a highly conserved Wnt‐transport protein, in rat tissues, zebrafish embryos, and cultured human cells
Author(s) -
Jin Jay,
Morse Megan,
Frey Colleen,
Petko Jessica,
Levenson Robert
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22369
Subject(s) - biology , zebrafish , in situ hybridization , wnt signaling pathway , transmembrane protein , embryo , microbiology and biotechnology , messenger rna , secretion , embryonic stem cell , vertebrate , embryogenesis , developmental biology , gene , genetics , signal transduction , endocrinology , receptor
GPR177 is an evolutionarily conserved transmembrane protein necessary for Wnt protein secretion. Little is currently known, however, regarding expression of GPR177, especially in vertebrate species. We have developed an antiserum against GPR177, and used it to examine expression of GPR177 in human tissue culture cells, adult mouse, and rat tissues, as well as developing zebrafish embryos. In rodents, GPR177 is expressed in virtually all tissue types and brain regions examined. In zebrafish, GPR177 polypeptides are expressed throughout embryogenesis, and are detectable as early as 1 hr post‐fertilization. In situ hybridization analysis reveals that gpr 177 mRNA expression is prominent in embryonic zebrafish brain and ear. Structural studies suggest that GPR177 is modified by N‐linked sugars, and that the protein contains an even number of transmembrane segments. The relatively ubiquitous expression of GPR177 suggests that this protein may serve to regulate Wnt secretion in a variety of embryonic and adult tissue types. Developmental Dynamics 239:2426–2434, 2010. © 2010 Wiley‐Liss, Inc.