Premium
Identification of downstream targets of the Bone Morphogenetic Protein pathway in the Drosophila nervous system
Author(s) -
Kim Nam Chul,
Marqués Guillermo
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22368
Subject(s) - biology , bone morphogenetic protein , imaginal disc , nervous system , microbiology and biotechnology , gene , mutant , alternative splicing , signal transduction , microarray analysis techniques , drosophila melanogaster , g protein coupled receptor , genetics , gene isoform , gene expression , neuroscience
Bone Morphogenetic Protein (BMP) signaling mediated by the receptor Wishful thinking (Wit) is essential for nervous system development in Drosophila . Mutants lacking wit function show defects in neuromuscular junction development and function, specification of neurosecretory phenotypes, and eclosion behavior that result in lethality. The ligand is Glass bottom boat, the Drosophila ortholog of mammalian BMP‐7, which acts as a retrograde signal through the Wit receptor. In order to identify transcriptional targets of the BMP pathway in the Drosophila nervous system, we have analyzed the gene expression profile of wit mutant larval central nervous system. Genes differentially expressed identified by microarray analysis have been verified by quantitative PCR and studied by in situ hybridization. Among the genes thus identified, we find solute transporters, neuropeptides, mitochondrial proteins, and novel genes. In addition, several genes are regulated by wit in an isoform‐specific manner that suggest regulation of alternative splicing by BMP signaling. Developmental Dynamics 239:2413–2425, 2010. © 2010 Wiley‐Liss, Inc.