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Loss of procollagen IIA from the anterior mesendoderm disrupts the development of mouse embryonic forebrain
Author(s) -
Leung Alan W.L.,
Wong Sandra Y.Y.,
Chan Danny,
Tam Patrick P.L.,
Cheah Kathryn S.E.
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22366
Subject(s) - biology , endoderm , mesoderm , forebrain , microbiology and biotechnology , morphogenesis , sonic hedgehog , anatomy , embryonic stem cell , endocrinology , genetics , signal transduction , central nervous system , gene
Morphogenesis of the mammalian forebrain is influenced by the patterning activity of signals emanating from the anterior mesendoderm. In this study, we show that procollagen IIA (IIA), an isoform of the cartilage extracellular matrix protein encoded by an alternatively spliced transcript of Col2a1 , is expressed in the prechordal plate and the anterior definitive endoderm. In the absence of IIA activity, the null mutants displayed a partially penetrant phenotype of loss of head tissues, holoprosencephaly, and loss of mid‐facial structures, which is associated with reduced sonic hedgehog ( Shh ) expression in the prechordal mesoderm. Genetic interaction studies reveal that IIA function in forebrain and face development does not involve bone morphogenetic protein receptor 1A (BMPR1A)‐ or NODAL‐mediated signaling activity. Developmental Dynamics 239:2319–2329. © 2010 Wiley‐Liss, Inc.