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Cell type–specific expression of adenomatous polyposis coli in lung development, injury, and repair
Author(s) -
Li Aimin,
Xing Yiming,
Chan Belinda,
Heisterkamp Nora,
Groffen John,
Borok Zea,
Minoo Parviz,
Li Changgong
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22364
Subject(s) - biology , progenitor cell , wnt signaling pathway , adenomatous polyposis coli , microbiology and biotechnology , progenitor , lung , cancer research , morphogenesis , pathology , cell type , cell , stem cell , signal transduction , medicine , genetics , colorectal cancer , cancer , gene
Adenomatous polyposis coli (Apc) is critical for Wnt signaling and cell migration. The current study examined Apc expression during lung development, injury, and repair. Apc was first detectable in smooth muscle layers in early lung morphogenesis, and was highly expressed in ciliated and neuroendocrine cells in the advanced stages. No Apc immunoreactivity was detected in Clara or basal cells, which function as stem/progenitor cell in adult lung. In ciliated cells, Apc is associated mainly with apical cytoplasmic domain. In response to naphthalene‐induced injury, Apc positive cells underwent squamous metaplasia, accompanied by changes in Apc subcellular distribution. In conclusion, both spatial and temporal expression of Apc is dynamically regulated during lung development and injury repair. Differential expression of Apc in progenitor vs. nonprogenitor cells suggests a functional role in cell‐type specification. Subcellular localization changes of Apc in response to naphthalene injury suggest a role in cell shape and cell migration. Developmental Dynamics 239:2288–2297, 2010. © 2010 Wiley‐Liss, Inc.