Premium
In vitro and in vivo characterization of Caenorhabditis elegans PHA‐4/FoxA response elements
Author(s) -
Raharjo Wahyu Hendrati,
Logan Brittany C.,
Wen Shoubin,
Kalb John M.,
Gaudet Jeb
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22359
Subject(s) - biology , caenorhabditis elegans , transcription factor , genetics , gene , microbiology and biotechnology , in vivo , caenorhabditis , transcription (linguistics) , promoter , reporter gene , in vitro , consensus sequence , gene expression , peptide sequence , linguistics , philosophy
Caenorhabditis elegans PHA‐4 is a member of the FoxA group of transcription factors. PHA‐4 is critical for development of the C. elegans pharynx and directly regulates most or all pharyngeal genes. The consensus binding site of PHA‐4 has not been identified, with previous analysis of PHA‐4 targets relying on the mammalian FoxA consensus. Here, we use in vitro and in vivo analyses to demonstrate three features of PHA‐4 response elements. First, the PHA‐4 consensus matches that of other FoxA proteins, but only a subset of possible sites is active in an in vivo assay. Second, sequence flanking the core PHA‐4 site can influence the strength of reporter expression in vivo, as seen for other Fox proteins. Third, in the context of some pharyngeal promoters, PHA‐4 response elements are flanked by distinct cis ‐regulatory elements that modulate response to PHA‐4, generating gene expression in specific pharyngeal cell types. Developmental Dynamics 239:NA–NA, 2010. © 2010 Wiley‐Liss, Inc.