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Evidence of interlobular repulsion during branching morphogenesis in mouse salivary glands
Author(s) -
Okamoto Katsuya,
KikuchiHanda Tomomi,
Nogawa Hiroyuki
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22354
Subject(s) - biology , morphogenesis , microbiology and biotechnology , bromodeoxyuridine , hedgehog , fibroblast growth factor , developmental biology , anatomy , immunology , receptor , signal transduction , genetics , immunohistochemistry , gene
Abstract We developed a culture method for detecting repulsion among epithelial lobules during branching morphogenesis in mouse submandibular glands. Three epithelia were placed at each vertex of an imaginary triangle apart but near enough to meet with one another if each of them expands radially during the culture period. No repulsion was observed following cultivation with neuregulin 1 and lysophosphatidic acid; the epithelia actively branched and nearly contacted one another in the triangle's center. In contrast, strong repulsion was observed among the epithelia cultured with fibroblast growth factor 1 (FGF1), which exhibited less branching and moved away from the center. The localization of DiI‐ (1,1′, di‐octadecyl‐3,3,3′,3′,‐tetramethylindo‐carbocyanine perchlorate) and BrdU‐ (5‐bromodeoxyuridine) labeled cells in the cultures exposed to FGF1 indicated that the cells were unable to move and proliferate in the center. SB431542, an inhibitor of transforming growth factor‐β (TGFβ) signaling, was unable to abolish this repulsion, suggesting that TGFβs will not probably act as repellants in this case. Developmental Dynamics 239:2208–2218, 2010. © 2010 Wiley‐Liss, Inc.