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Cellular dynamics of epithelial clefting during branching morphogenesis of the mouse submandibular gland
Author(s) -
Kadoya Yuichi,
Yamashina Shohei
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22312
Subject(s) - biology , morphogenesis , microfilament , microbiology and biotechnology , epithelium , anatomy , cell junction , submandibular gland , cell , cytoskeleton , endocrinology , biochemistry , genetics , gene
We cultured the rudimental submandibular gland (SMG) of mice with a non–cell‐permeable fluorescent tracer, and observed cell behavior during epithelial branching morphogenesis using confocal time‐lapse microscopy. We traced movements of individual cells as shadowgraph movies. Individual epithelial cells migrated dynamically but erratically. The epithelial cleft extended by wiggling and separated a cluster of cells into two buds during branching. We examined the ultrastructure of the clefts in SMG rudiments treated with the laminin peptide A5G77f, which induces epithelial clefting. A short cytoplasmic shelf with a core of microfilaments was found at the deep end of the cleft. We propose that epithelial clefting involves a dynamic movement of cells at the base of the cleft, and the formation of a shelf within a cleft cell. The shelf might form a matrix attachment point at the base of the cleft with a core of microfilaments driving cleft elongation. Developmental Dynamics 239:1739–1747, 2010. © 2010 Wiley‐Liss, Inc.