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Expression of strawberry notch family genes during zebrafish embryogenesis
Author(s) -
Takano Ai,
Zochi Riyo,
Hibi Masahiko,
Terashima Toshio,
Katsuyama Yu
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22287
Subject(s) - biology , zebrafish , embryogenesis , gene , in situ hybridization , embryo , neural development , gene expression , central nervous system , genetics , nervous system , microbiology and biotechnology , gene family , neuroscience
Our previous study suggested a possible role for Sbno1 , a mouse homologue of strawberry notch gene during brain development. In this report, we cloned the zebrafish homologues of sbno , and examined their expression pattern during embryogenesis by whole‐mount in situ hybridization. Zebrafish have three sbno genes: one Sbno1 homologue and two Sbno2 homologues, sbno2a and sbno2b . We observed that the expression of sbno1 and sbno2a was initially ubiquitous and gradually became predominant in the central nervous system as development progressed. The expression of sbno2b was observed in non‐neural tissues in contrast to the other two genes. sbno1 and sbno2a exhibited higher expression in distinct regions within the nervous system of pharyngula‐stage embryos, suggesting possible differing roles for sbno1 and sbno2a during later stages of embryogenesis. Together, the observed gene expression patterns suggest an important role of sbno ‐family genes during development of the vertebrate central nervous system. Developmental Dynamics 239:1789–1796, 2010. © 2010 Wiley‐Liss, Inc.