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Prickle1b mediates interpretation of migratory cues during zebrafish facial branchiomotor neuron migration
Author(s) -
Mapp Oni M.,
Wanner Sarah J.,
Rohrschneider Monica R.,
Prince Victoria E.
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22283
Subject(s) - biology , zebrafish , neuroscience , wnt signaling pathway , hindbrain , cell polarity , centrosome , cell migration , vertebrate , neuron , microbiology and biotechnology , anatomy , signal transduction , cell , central nervous system , genetics , cell cycle , gene
The facial branchiomotor neurons undergo a characteristic tangential migration in the vertebrate hindbrain. Several signaling mechanisms have been implicated in this process, including the non‐canonical Wnt/planar cell polarity (PCP) pathway. However, the role of this signaling pathway in controlling the dynamics of these neurons is unclear. Here, we describe the cellular dynamics of the facial neurons as they migrate, focusing on the speed and direction of migration, extension of protrusions, cell shape, and orientation. Furthermore, we show that the PET/LIM domain protein Prickle1b (Pk1b) is required for several aspects of these migratory behaviors, including cell orientation. However, we find that centrosome localization is not significantly affected by disruption of Pk1b function, suggesting that polarization of the neurons is not completely lost. Together, our data suggest that Pk1b function may be required to integrate the multiple migratory cues received by the neurons into polarization instructions for proper posterior movement. Developmental Dynamics 239:1596–1608, 2010. © 2010 Wiley‐Liss, Inc.