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Genetic network underlying temperature‐dependent sex determination is endogenously regulated by temperature in isolated cultured Trachemys scripta gonads
Author(s) -
ShoemakerDaly Christina M.,
Jackson Kyle,
Yatsu Ryohei,
Matsumoto Yuiko,
Crews David
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22266
Subject(s) - biology , sox9 , gonad , embryonic stem cell , turtle (robot) , microbiology and biotechnology , incubation , sexual differentiation , development of the gonads , zoology , genetics , andrology , anatomy , gene , gene expression , ecology , biochemistry , medicine
In reptiles with temperature‐dependent sex determination, gonadogenesis is initially directed by the incubation temperature of the egg during the middle third of embryonic development. The mechanism by which temperature is transduced into a sex‐determining molecular signal remains a mystery, and here we examine the molecular network underlying sex determination in gonads in vitro. We use a whole organ culture system to show that expression of putative members of the sex‐determining network ( Dmrt1 , Sox9 , Mis , and FoxL2 ) are regulated by temperature endogenously within cells in the bipotential gonad and do not require other embryonic tissues to be expressed in a normal pattern in the red‐eared slider turtle, Trachemys scripta . Furthermore, following a change in temperature, these factors exhibit temperature‐responsive expression patterns that last for the duration of gonadogenesis. Finally, mosaic misexpression of a fusion Sox9 construct demonstrates the ability to functionally manipulate the gonad at the molecular level. Developmental Dynamics 239:1061–1075, 2010. © 2010 Wiley‐Liss, Inc.