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Ror2 is required for midgut elongation during mouse development
Author(s) -
Yamada Makiko,
Udagawa Jun,
Matsumoto Akihiro,
Hashimoto Ryuju,
Hatta Toshihisa,
Nishita Michiru,
Minami Yasuhiro,
Otani Hiroki
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22212
Subject(s) - midgut , biology , endoderm , morphogenesis , microbiology and biotechnology , embryo , embryogenesis , wnt signaling pathway , embryonic stem cell , phenotype , organogenesis , mesoderm , anatomy , signal transduction , genetics , gene , botany , larva
The receptor tyrosine kinase Ror2 acts as a receptor for Wnt5a to mediate noncanonical Wnt signaling, and it plays essential roles in morphogenesis. Ror2 −/− embryos exhibit phenotypes similar to, albeit generally milder than, those of Wnt5a −/− embryos. During mouse embryogenesis, Ror2 is expressed in various organs and regions, although little is known about its expression pattern and roles in the developing gut, while Wnt5a is expressed in the developing gut, where its absence causes abnormal phenotypes. Here, we demonstrated that Ror2 was strongly and differentially expressed in the rostral and middle midgut endoderm from embryonic day (E) 10.5 through embryonic day (E) 12.5. At E11.5, Ror2 −/− embryos exhibited a shorter middle midgut with a larger diameter and more accumulation of epithelial cells in the middle midgut than control embryos, while the total cell numbers remained unaltered. These findings suggest that Ror2 plays important roles in midgut elongation by means of an epithelial convergent extension mechanism. Developmental Dynamics 239:941–953, 2010. © 2010 Wiley‐Liss, Inc.