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Expression and regulation of ANTXR1 in the chick embryo
Author(s) -
Herrmann David,
FerrerVaquer Anna,
Lahsnig Christian,
Firnberg Nicole,
Leibbrandt Andreas,
Neubüser Annette
Publication year - 2010
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22194
Subject(s) - biology , microbiology and biotechnology , mesenchyme , morphogenesis , frizzled , fibroblast growth factor , cadherin , wnt signaling pathway , embryo , receptor , gene , genetics , signal transduction , cell
Abstract Anthrax Toxin Receptor 1 ( ANTXR1 ; also known as Tumor Endothelial Marker 8 , TEM8 ) is one of several genes that was recently found to be up‐regulated in tumor‐associated endothelial cells. In vitro, the protein can link extracellular matrix components with the actin cytoskeleton to promote cell adhesion and cell spreading. Both, ANTXR1 and the closely related ANTXR2 can bind anthrax toxin and interact with lipoprotein receptor‐related protein 5 and 6, which also work as coreceptors in the WNT signaling pathway. Here, we report the cloning of chick ANTXR1 from a suppression subtractive hybridization screen for fibroblast growth factor (FGF) ‐inducible genes in chicken embryonic facial mesenchyme. We show that chicken ANTXR1 is dynamically expressed throughout embryogenesis, starting from Hamburger and Hamilton stage 10. Furthermore, we demonstrate that FGF signaling is sufficient, but not necessary, to induce ANTXR1 expression in chicken facial mesenchyme. Developmental Dynamics 239:680–687, 2010. © 2009 Wiley‐Liss, Inc.