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Enucleation of feeder cells and egg cells with psoralens
Author(s) -
McGarry Thomas J.,
Bonaguidi Michael,
Lyass Ljuba,
Kessler John A.,
Bodily Jason M.,
Doglio Lynn
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22177
Subject(s) - enucleation , biology , blastula , xenopus , green fluorescent protein , anatomy , microbiology and biotechnology , gastrulation , embryo , embryogenesis , genetics , gene
GFP‐expressing Xenopus tadpole made by transplanting a nucleus from a transgenic CMV‐GFP blastula into an enucleated GFP(−) egg. The egg was enucleated using a new method, treatment with psoralens and ultraviolet light. See McGarry et al., Developmental Dynamics 238:2614–2621, 2009. © 2009 Wiley‐Liss, Inc.