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Brn3a regulates the transition from neurogenesis to terminal differentiation and represses non‐neural gene expression in the trigeminal ganglion
Author(s) -
Lanier Jason,
Dykes Iain M.,
Nissen Stephanie,
Eng S. Raisa,
Turner Eric E.
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22145
Subject(s) - biology , trigeminal ganglion , neurogenesis , neuroscience , spinal trigeminal nucleus , microbiology and biotechnology , hindbrain , transcription factor , sensory system , psychological repression , neural development , neural crest , anatomy , gene expression , genetics , gene , embryo , nociception , central nervous system , receptor
The POU‐domain transcription factor Brn3a is expressed in developing sensory neurons at all levels of the neural axis, including the trigeminal ganglion, hindbrain sensory ganglia, and dorsal root ganglia. Changes in global gene expression in the trigeminal ganglion from E11.5 to E13.5 reflect the repression of early neurogenic genes, exit from the cell cycle, and initiation of the expression of definitive markers of sensory function. A majority of these developmental changes are perturbed in the trigeminal ganglia of Brn3a knockout mice. At E13.5, Brn3a −/− trigeminal neurons fail to repress a battery of developmental regulators that are highly expressed at E11.5 and are normally down‐regulated as development progresses, and also fail to appropriately activate a set of definitive sensory genes. Remarkably, developing Brn3a −/− trigeminal neurons also ectopically express multiple regulatory genes associated with cardiac and/or cranial mesoderm development, although definitive myogenic programs are not activated. The majority of these genes are not ectopically expressed in the dorsal root ganglia of Brn3a null mice, perhaps due to redundant mechanisms of repression at spinal levels. These results underscore the importance of gene repression in regulating neuronal development, and the need for unbiased screens in the determination of developmental gene regulatory programs. Developmental Dynamics 238:3065–3079, 2009. © 2009 Wiley‐Liss, Inc.