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Characterization of p70 S6 kinase 1 in early development of mouse embryos
Author(s) -
Xu XiaoYan,
Zhang Zhe,
Su WenHui,
Zhang Yang,
Yu YanQiu,
Li YanXiao,
Zong ZhiHong,
Yu BingZhi
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22131
Subject(s) - p70 s6 kinase 1 , mtorc1 , biology , mtorc2 , pi3k/akt/mtor pathway , microbiology and biotechnology , signal transduction
The mTOR kinase controls cell growth, proliferation, and survival through two distinct multiprotein complexes mTORC1 and mTORC2. p70 S6 Kinase 1 (S6K1) is characterized as downstream effector of mTOR. Until recently, the connection between S6K1 and mTORC1 /mTORC2 during the early development of mouse embryos has not been well elucidated. Here, the expression level of total S6K1 and its phosphorylation at Thr389 was determined in four phases of one‐cell embryos. S6K1 was active throughout the cell cycle especially with higher activity in G2 and M phases. Rapamycin decreased the activity of M‐phase promoting factor (MPF) and delayed the first mitotic cleavage. Down‐regulating mTOR and raptor reduced S6K1 phosphorylation at Thr389 in one‐cell embryos. Furthermore, rapamycin and microinjection of raptor shRNA decreased the immunofluorescent staining of Thr389 phospho‐S6K1. It is proposed that mTORC1 may be involved in the control of MPF by regulating S6K1 during the early development of mouse embryos. Developmental Dynamics 238:3025–3034, 2009. © 2009 Wiley‐Liss, Inc.