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Polarized expression of integrin β1 in diencephalic roof plate during chick development, a possible receptor for SCO‐spondin
Author(s) -
Caprile Teresa,
Osorio Germán,
Henríquez Juan Pablo,
Montecinos Hernán
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22070
Subject(s) - biology , integrin , extracellular matrix , subcommissural organ , microbiology and biotechnology , basement membrane , diencephalon , floor plate , secretion , anatomy , receptor , neuroscience , endocrinology , central nervous system , genetics
The roof plate of the caudal diencephalon is formed by the posterior commissure (PC) and the underlying secretory ependyma, the subcommissural organ (SCO). The SCO is composed by radial glial cells bearing processes that cross the PC and attach to the meningeal basement membrane. Since early development, the SCO synthesizes SCO‐spondin, a glycoprotein that shares similarities to axonal guidance proteins. In vitro, SCO‐spondin promotes neuritic outgrowth through a mechanism mediated by integrin β1. However, the secretion of SCO‐spondin toward the extracellular matrix that surrounds the PC axons and the expression of integrins throughout PC development have not been addressed. Here we provide immunohistochemical evidence to suggest that during chick development SCO cells secrete SCO‐spondin through their basal domain, where it is deposited into the extracellular matrix in close contact with axons of the PC that express integrin β1. Our results suggest that SCO‐spondin has a role in the development of the PC through its interaction with integrin β1. Developmental Dynamics 238:2494–2504, 2009. © 2009 Wiley‐Liss, Inc.