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Cell‐autonomous requirements for Dlg‐1 for lens epithelial cell structure and fiber cell morphogenesis
Author(s) -
Rivera Charlene,
Yamben Idella F.,
Shatadal Shalini,
Waldof Malinda,
Robinson Michael L.,
Griep Anne E.
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.22036
Subject(s) - microbiology and biotechnology , biology , morphogenesis , cell polarity , cell adhesion , cell , lens (geology) , focal adhesion , stress fiber , cytoskeleton , signal transduction , genetics , gene , paleontology
Cell polarity and adhesion are thought to be key determinants in organismal development. In Drosophila , discs large ( dlg ) has emerged as an important regulator of epithelial cell proliferation, adhesion, and polarity. Herein, we investigated the role of the mouse homolog of dlg ( Dlg‐1 ) in the development of the mouse ocular lens. Tissue‐specific ablation of Dlg‐1 throughout the lens early in lens development led to an expansion and disorganization of the epithelium that correlated with changes in the distribution of adhesion and polarity factors. In the fiber cells, differentiation defects were observed. These included alterations in cell structure and the disposition of cell adhesion/cytoskeletal factors, delay in denucleation, and reduced levels of α‐catenin, pERK1/2, and MIP26. These fiber cell defects were recapitulated when Dlg‐1 was disrupted only in fiber cells. These results suggest that Dlg‐1 acts in a cell autonomous manner to regulate epithelial cell structure and fiber cell differentiation. Developmental Dynamics 238:2292–2308, 2009. © 2009 Wiley‐Liss, Inc.