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High resolution fate map of the zebrafish diencephalon
Author(s) -
RussekBlum Niva,
NabelRosen Helit,
Levkowitz Gil
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21987
Subject(s) - diencephalon , zebrafish , biology , fate mapping , neuroscience , floor plate , carassius auratus , morphogenesis , neural tube , vertebrate , anatomy , microbiology and biotechnology , progenitor cell , embryo , fish <actinopterygii> , central nervous system , gene , genetics , stem cell , fishery
The diencephalon acts as an interactive site between the sensory, central, and endocrine systems and is one of the most elaborate structures in the vertebrate brain. To better understand the embryonic development and morphogenesis of the diencephalon, we developed an improved photoactivation (uncaging)‐based lineage tracing strategy. To determine the exact position of a given diencephalic progenitor domain, we used a transgenic line driving green fluorescent protein (GFP) in cells expressing the proneural protein, Neurogenin1 (Neurog1), which was used as a visible neural plate landmark. This approach facilitated precise labeling of defined groups of cells in the prospective diencephalon of the zebrafish neural plate. In this manner, we labeled multiple overlapping areas of the diencephalon, thereby ensuring both accuracy and reproducibility of our lineage tracing regardless of the dynamic changes of the developing neural plate. We present a fate map of the zebrafish diencephalon at a higher spatial resolution than previously described. Developmental Dynamics 238:1827–1835, 2009. © 2009 Wiley‐Liss, Inc.