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Zebrafish wnt3 is expressed in developing neural tissue
Author(s) -
Clements Wilson K.,
Ong Karen G.,
Traver David
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21977
Subject(s) - biology , zebrafish , hindbrain , mesoderm , wnt signaling pathway , microbiology and biotechnology , forebrain , neural development , neuroectoderm , neural crest , neuroscience , anatomy , embryonic stem cell , genetics , embryo , central nervous system , signal transduction , gene
Wnt signaling regulates embryonic patterning and controls stem cell homeostasis, while aberrant Wnt activity is associated with disease. One Wnt family member, Wnt3, is required in mouse for specification of mesoderm, and later regulates neural patterning, apical ectodermal ridge formation, and hair growth. We have identified and performed preliminary characterization of the zebrafish wnt3 gene. wnt3 is expressed in the developing tailbud and neural tissue including the zona limitans intrathalamica (ZLI), optic tectum, midbrain‐hindbrain boundary, and dorsal hindbrain and spinal cord. Expression in these regions suggests that Wnt3 participates in processes such as forebrain compartmentalization and regulation of tectal wiring topography by retinal ganglia axons. Surprisingly, wnt3 expression is not detectable during mesoderm specification, making it unlikely that Wnt3 regulates this process in zebrafish. This lack of early expression should make it possible to study later Wnt3‐regulated patterning events, such as neural patterning, by knockdown studies in zebrafish. Developmental Dynamics 238:1768–1795, 2009. © 2009 Wiley‐Liss, Inc.