Premium
Rapid gynogenetic mapping of Xenopus tropicalis mutations to chromosomes
Author(s) -
Khokha Mustafa K.,
Krylov Vladimir,
Reilly Michael J.,
Gall Joseph G.,
Bhattacharya Dipankan,
Cheung Chung Yan J.,
Kaufman Sarah,
Lam Dang Khoa,
Macha Jaroslav,
Ngo Catherine,
Prakash Neha,
Schmidt Philip,
Tlapakova Tereza,
Trivedi Toral,
Tumova Lucie,
AbuDaya Anita,
Geach Timothy,
Vendrell Elisenda,
Ironfield Holly,
Sinzelle Ludivine,
Sater Amy K.,
Wells Dan E.,
Harland Richard M.,
Zimmerman Lyle B.
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21965
Subject(s) - biology , xenopus , genetics , centromere , gene mapping , fluorescence in situ hybridization , chromosome , computational biology , gene
Pilot forward genetic screens in Xenopus tropicalis have isolated over 60 recessive mutations. Here we present a simple method for mapping mutations to chromosomes using gynogenesis and centromeric markers. When coupled with available genomic resources, gross mapping facilitates evaluation of candidate genes as well as higher resolution linkage studies. Using gynogenesis, we have mapped the genetic locations of the 10 X. tropicalis centromeres, and performed fluorescence in situ hybridization to validate these locations cytologically. We demonstrate the use of this very small set of centromeric markers to map mutations efficiently to specific chromosomes. Developmental Dynamics 238:1398–1406, 2009. © 2009 Wiley‐Liss, Inc.