Premium
Formaldehyde‐based whole‐mount in situ hybridization method for planarians
Author(s) -
Pearson Bret J.,
Eisenhoffer George T.,
Gurley Kyle A.,
Rink Jochen C.,
Miller Diane E.,
Alvarado Alejandro Sánchez
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21937
Subject(s) - planarian , biology , in situ hybridization , anatomy , in situ , regeneration (biology) , immunofluorescence , microbiology and biotechnology , messenger rna , genetics , antibody , physics , meteorology , gene
Robust, high resolution formaldehyde‐based in situ hybridization method for the interrogation of planarian regeneration. Schmidtea mediterranea specimen after head and tail regeneration labeled by combining immunofluorescence and double fluorescent in situ hybridization. Riboprobes for Smed ‐ porcn ‐ 1 (gastrovascular system) and Smed‐slit (midline cells) are shown in blue and green, respectively, while mouse anti‐a‐tubulin antibody (brain, ventral nerve chords, pharynx, flame cells) is shown in red/magenta. Images obtained by Sarah Elliott and Kyle Gurley, University of Utah. See Pearson et al., Developmental Dynamics 238:443‐450. Developmental Dynamics, 2009. © 2009 Wiley‐Liss, Inc.