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c ‐ fos elimination compensates for disabled ‐ 2 requirement in mouse extraembryonic endoderm development
Author(s) -
Yang DongHua,
Smith Elizabeth R.,
Cai Kathy Qi,
Xu XiangXi
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21856
Subject(s) - endoderm , biology , organogenesis , microbiology and biotechnology , embryo , embryonic stem cell , embryogenesis , genetics , gene
Disabled‐2 (Dab2) is expressed in primitive endoderm cells as they are differentiating from the inner cell mass and dab2 deficiency in mice results in lethality at E5.5–E6.5 due to the disorganization of the endoderm layers. Here we show that Dab2 suppresses c‐Fos expression in endoderm cells. A morphological normal primitive endoderm layer was observed in putative E5.5 dab2 (−/−): c ‐ fos (−/−) embryos, indicating that the primitive endoderm defect due to the loss of Dab2 is rescued by deletion of the c ‐ fos gene. The lethality of the double knockout embryos was delayed until E9.5–E10.5 and the defective embryos failed to undergo organogenesis. We conclude that Dab2 plays a role in epithelial organization by suppression of c‐Fos expression and suggest that unsuppressed c‐Fos can lead to disruption of primitive endoderm epithelial organization, yet an additional dab2 function is required for later organogenesis. Developmental Dynamics 238:514–523, 2009. © 2009 Wiley‐Liss, Inc.