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gfap and nestin reporter lines reveal characteristics of neural progenitors in the adult zebrafish brain
Author(s) -
Lam Chen Sok,
März Martin,
Strähle Uwe
Publication year - 2009
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.21853
Subject(s) - nestin , biology , zebrafish , neural stem cell , neurogenesis , glial fibrillary acidic protein , microbiology and biotechnology , colocalization , sox2 , neurosphere , transgene , progenitor cell , green fluorescent protein , stem cell , cellular differentiation , adult stem cell , immunohistochemistry , genetics , transcription factor , immunology , gene
Adult neurogenesis arises from niches that harbor neural stem cells (NSC). Although holding great promise for regenerative medicine, the identity of NSC remains elusive. In mammals, a key attribute of NSC is the expression of the filamentous proteins glial fibrillary acidic protein (GFAP) and NESTIN. To assess whether these two markers are relevant in the fish model, two transgenic zebrafish lines for gfap and nestin were generated. Analysis of adult brains showed that the fusion GFAP–green fluorescent protein closely mimics endogenous GFAP, while the nestin transgene recapitulates nestin at the ventricular zones. Cells expressing the two reporters display radial glial morphology, colocalize with the NSC marker Sox2, undergo proliferation, and are capable of self‐renewal within the matrix of distinct thickness in the telencephalon. Together, these two transgenic lines reveal a conserved feature of putative NSC in the adult zebrafish brain and provide a means for the identification and manipulation of these cells in vivo. Developmental Dynamics 238:475–486, 2009. © 2009 Wiley‐Liss, Inc.